Collecting X-ray Powder Diffraction Data: how to choose the best configuration and measurement parameters – 22 May 2018

X-ray Powder Diffractometers are versatile instruments that often come with a variety of optics and accessories that can be exchanged to optimize measurements. While these options provide flexibility, they can also leave one unsure about the best configuration to use. This presentation will teach ways to navigate all of the choices and select the best configuration and measurement parameters to collect powder diffraction data from a variety of samples.

This presentation will:

  • review the common optics used for X-ray powder diffraction and discuss their impact on data quality;
  • discuss how configuration affects resolution, intensity, beam size, etc;
  • identify the most critical criteria for collecting accurate data;
  • discuss how different sample types (clinker, mining, chemical, pharmaceutical, advanced materials) place different requirements on measurements;
  • discuss strategies for optimizing measurement parameters.

Summary

Date:
May 22 2018 – May 22 2018
Time:
10:30 – 11:30
(GMT-05:00) Eastern [US & Canada]
Event type:
Webinar – Live
Language:
English
Products:
X’Pert3 range
Technology:
X-ray Diffraction (XRD)

Speakers

Scott Speakman, XRD Principal Scientist at Malvern Panalytical

More information

Why attend?
Attendees will leave with practical knowledge on selecting the optimal instrument set up to obtain the best data for a certain type of sample.

What will you learn?
XRD system components and their purposes and affects on the quality of data. Strategies for best setups for given sample types. As a bonus they will be able to download a chart from the presenter that makes setting up experiments for different samples effortless.

For more information and to register please CLICK HERE

Enlightening the Fcab-antigen interaction via X-ray crystallography & solution studies – 15 May 2018

The modular nature of antibodies allows the design of antibody-related formats with tailored characteristics. The antibody’s Fc part, encompassing the effector functions, represents an attractive scaffold for engineering therapeutic molecules as demonstrated by the generation of Fcabs (Fc domain with antigen-binding sites). By engineering the C-terminal loops (AB, CD and EF loop) in the CH3 domains, two antigen binding sites can be inserted in close proximity.

The study presented in this webinar deals with the determination of the first X-ray structures of three Fcabs that differ in loop design, specificity, affinity and thermal stability and reveals their overall structural integrity and native fold.

Furthermore, by investigation of the interaction of these Fcabs with their respective antigens (HER2 or VEGF) using a set of complementary techniques (X-ray crystallography, size exclusion chromatography combined with multi-angle light scattering [SEC-MALS], isothermal titration calorimetry [ITC], fluorescence correlation spectroscopy [FCS]), insights into the binding modes and binding stoichiometries were gained.

Summary

Date:
May 15 2018 – May 15 2018
Time:
10:30 – 11:30
(GMT-05:00) Eastern [US & Canada]
Event type:
Webinar – Live
Language:
English
Technology:
Size Exclusion Chromatography (SEC)
X-ray Diffraction (XRD)
Isothermal Titration Calorimetry (ITC)
Industry:
Biosciences

Who should attend?
Everyone who focuses on antibodies and antibody fragments and is interested in state-of-the-art biophysical methods to analyze protein-protein interactions.

What will you learn?
The presented study demonstrates how to apply and exploit complementary biophysical methods to shed light on protein-protein interactions.

For registration and more information please CLICK HERE